Expression of the matrix metalloproteinase promatrilysin in coculture of prostate carcinoma cell lines

Citation
Ms. Stratton et al., Expression of the matrix metalloproteinase promatrilysin in coculture of prostate carcinoma cell lines, PROSTATE, 48(3), 2001, pp. 206-209
Citations number
18
Categorie Soggetti
Urology & Nephrology","da verificare
Journal title
PROSTATE
ISSN journal
02704137 → ACNP
Volume
48
Issue
3
Year of publication
2001
Pages
206 - 209
Database
ISI
SICI code
0270-4137(20010801)48:3<206:EOTMMP>2.0.ZU;2-F
Abstract
BACKGROUND. Matrix metalloproteinases (MMPs) are involved in tumor progress ion. Matrilysin (MMP-7) has been shown to be upregulated in prostatic carci nomas and can increase the invasive capacity of DU-145 cells. Because of th e heterogenous nature of prostatic tumors, we examined promatrilysin expres sion in cocultures containing two different prostatic carcinoma cell lines, DU-145 and LNCaP. METHODS. Using enzyme linked immunosorbent assay (ELISA) analyses, promatri lysin expression was measured in DU-145/LNCaP cocultures and conditioned me dia cross-cultures. The effects of blocking IL-6 on promatrilysin expressio n were examined by pretreating conditioned media with IL-6 neutralizing ant ibody. RESULTS. A significant induction of promatrilysin expression was observed i n DU-145/LNCaP cocultures compared to LNCaP cells alone. In addition, DU-14 5 conditioned medium induced the same fold induction of promatrilysin as wa s observed in the cocultures. LNCaP cell conditioned medium did not induce promatrilysin expression in DU-145 cells. Neutralization of IL-6 with neutr alizing antibody abrogated DU-145 conditioned media induced promatrilysin e xpression to baseline levels. CONCLUSIONS. IL-6 secreted by DU-145 cells can induce promatrilysin express ion in LNCaP cells. IL-6, in vivo, may act as a paracrine signaling factor that regulates matrix metalloproteinase expression. Therefore, IL-6 may pla y a role in invasive metastatic processes of a prostate carcinoma.