Cloning of 87 kDa outer membrane protein gene of Pasteurella multocida p52

Pasteurella multocida serotype B:2 is the causative agent of haemorrhagic s epticaemia, a fatal disease of cattle and buffaloes. Formalin inactivated w hole cell bacterin is used to prepare vaccines in India. However, outer mem brane proteins (OMPS) of P multocida were reported to be potential immunoge ns. The 87-kDa omp of P multocida P52, serotype B:2 has been identified as one of the major antigens because this protein reacted with serum of vaccin ated animals. The gene omp87, encoding an 87-kDa omp was amplified and clon ed into pBluescript SK(-) vector. This gene was found to localise in a 9.0 kb Hind III fra.-ment of P multocida genome. (C) 2001 Harcourt Publishers L td.