Biliary organic anion excretion is mediated by an ATP-dependent primary act
ive transporter, multidrug resistance protein 2. On the other hand, a multi
plicity of canalicular organic anion transport has been suggested. Ursodeox
ycholic acid, the 7 beta -epimer of chenodeoxycholic acid, is clinically us
ed for various hepatobiliary diseases. In our previous study, the contribut
ion of multidrug resistance protein 2 for biliary excretion of taurine-conj
ugated bile acid sulfates depended on the numbers of hydroxyl residue. Ther
efore, to further examine the effect of hydrophobicity on the substrate spe
cificity of multidrug resistance protein 2, we examined the effect of bile
acid conjugates and organic anions on biliary excretion of tauroursodeoxych
olate-3-sulfate, taurine and sulfonate-conjugated ursodeoxycholic acid, in
rats. Biliary tauroursodeoxycholate-3-sulfate excretions was markedly delay
ed in Eisai hyperbilirubinemic rats. Taurolithocholate-3-sulfate inhibited
but ursodeoxycholate-3,7-disulfate did not affect biliary tauroursodeoxycho
late-3-sulfate excretion. Biliary tauroursodeoxycholate-3-sulfate excretion
was inhibited by sulfobromophthalein, but was not inhibited by dibromosulf
ophthalein and cefpiramide. These findings indicate that tauroursodeoxychol
ate-3-sulfate is very specific for multidrug resistance protein 2. (C) 2001
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