The 4-oxopentyl group as a labile phosphate/thiophosphate protecting groupfor synthetic oligodeoxyribonucleotides

Authors
Wilk, A Chmielewski, MK Grajkowski, A Phillips, LR Beaucage, SL
Citation
A. Wilk et al., The 4-oxopentyl group as a labile phosphate/thiophosphate protecting groupfor synthetic oligodeoxyribonucleotides, TETRAHEDR L, 42(33), 2001, pp. 5635-5639
Citations number
12
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON LETTERS
ISSN journal
00404039 → ACNP
Volume
42
Issue
33
Year of publication
2001
Pages
5635 - 5639
Database
ISI
SICI code
0040-4039(20010813)42:33<5635:T4GAAL>2.0.ZU;2-S
Abstract
An efficient and economical method for the solid-phase synthesis of oligode oxyribonucleotides and their phosphorothioate analogues is described. The m ethod entails the use of the 4-oxopentyl group for phosphate thiophosphate protection. Post-synthesis removal of the protecting group is easily and ra pidly achieved under mild conditions at ambient temperature using either pr essurized gaseous amines or concentrated ammonium hydroxide. (C) 2001 Elsev ier Science Ltd. All rights reserved.