The interaction between ctDNA and multi-pedal amino-compound with naphtholgroups

A series of multi - pedal amino - compound with naphthol groups have been s ynthesized in this work. The photo - physical behaviors of these compounds in different solvents and different pH conditions have also been studied in details. The properties of environment in which the probe molecule located were estimated properly by the ratio of emission intensity of naphthol and phenol anion peak wave - length in the fluorescence spectra of these compo unds. The problem of fluorescence quenching of the studied compounds by ctD NA was also been investigated. The results indicate that the compound with tripedal has a stronger interaction with ctDNA than that of other compounds for which one or two pedals were contained. The intercalation of naphthol group into double helix structure of ctDNA resulted in fluorescence quenchi ng of naphthol group due to the interaction of this group and the base pair of DNA. It is due to the fact that the dual fluorescence emission of excit ed naphthol group had different quenching constants by ctDNA. The mechanism of this interesting phenomenon was discussed preliminarily.