The aim of this study was to determine temporal and spatial variation of ai
rborne concentrations of viable fungi in 2 single-family houses during the
period of the year that such levels are mostly determined by indoor sources
. One of the subject residences had moisture problems (the index residence)
, the other did not have any moisture problems (the reference residence). T
he concentrations of viable fungi in indoor air were determined a total of
6 times during the winter with 2 six-stage impactors (Andersen 10-800) in 2
rooms in both of the houses. The total concentrations of viable fungi and
concentrations of Penicillium, Aspergillus, and Aspergillus versicolor were
significantly higher in the index residence than in the reference residenc
e. Statistically significant differences in the total concentrations were o
bserved in the size ranges of 2.1-3.3 mum and 1.1-2.1 mum. In both residenc
es, there was a statistically significant difference between the rooms in t
he total concentrations of viable fungi, although in the reference residenc
e that was not of practical value because the levels were low. In concentra
tions of fungal genera, a significant difference, that was also of practica
l importance, was seen in the concentrations of Penicillium in the index re
sidence. Both within-day and within-season differences of concentrations we
re observed in the residences: a trend of higher values in the morning and
at the beginning of winter was noted. In order to characterize fungal conce
ntrations of a residence with unknown indoor conditions, a sampling campaig
n of I I different days is needed. A strategy for responding to an alert or
alarm situation is presented.