ANTIOXIDATIVE ACTIVITY OF POLYPHENOL EXTR ACTS FROM BARLEY BRAN

Antioxidative activity of alcoholic extracts of polyphenols from barle y bran (polyphenol extracts)was studied. The extracts were prepared by following procedures. After removal of lipid by n-hexane from barley bran, it was extracted with 75% ethanol. The crude extracted solution was washed and purified by a Sepabeads SP -850 resin. This polyphenol fraction was eluted from the resin with 75% ethanol and evaporated und er 30 degrees C, and then freeze-dried. The polyphenol content of the extracts was 28.7% as (+)-catechin. Antioxidative activity of the poly phenol extracts to linoleic acid was found to be the same or less than that of BHA and BHT, but higher than that of dl-a-tocopherol and asco rbic acid by using the TEA and ferric thiocyanate methods. The polyphe nol extracts showed more effective super oxide scavenging ability than that of dl-alpha-tocopherol, BHA and BHT.