Requirements of Cyc2p and the porin, Por1p, for ionic stability and mitochondrial integrity in Saccharomyces cerevisiae

It was previously demonstrated that Cyc2p from Saccharomyces cerevisiae is a mitochondrial protein; that the cyc2-Delta2 deletion lacking the entire g ene causes a diminution to only approximately 20% of the normal levels of c ytochrome c due to a partial deficiency in mitochondrial import of apo-cyto chrome c; that the deletion causes a defective mitochondrial function, as r evealed by diminished growth on media containing nonfermentable carbon sour ces; and that this defect is exacerbated in hyper-ionic KCl media and at hi gher incubation temperatures, but is suppressed on media containing sorbito l, a nonionic compound. We report that por1-Delta strains lacking the mitoc hondrial porin, Por1p, but not por2-Delta strains lacking the related porin , share some phenotypes similar to the cyc2-Delta2 strain, including hypers ensitivity to KCl in glycerol medium. Moreover, spontaneous swelling in the presence of ATP was detected in mitochondria from the cyc2-Delta2 strain, while swelling could be detected in mitochondria from the other strains onl y after the addition of KCl. Thus, highly unspecific membrane permeation ma y be triggered by ATP in the cyc2-Delta2 strain. We suggest that Por1p and Cyc2p, in addition to their own unique functions, serve to maintain the osm otic stability of mitochondria, but by different mechanisms. (C) 2001 Acade mic Press.