Development of simple sequence repeat markers from bacterial artificial chromosomes without subcloning

Authors
Qi, X Lindup, S Pittaway, TS Allouis, S Gale, MD Devos, KM
Citation
X. Qi et al., Development of simple sequence repeat markers from bacterial artificial chromosomes without subcloning, BIOTECHNIQU, 31(2), 2001, pp. 355
Citations number
10
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOTECHNIQUES
ISSN journal
07366205 → ACNP
Volume
31
Issue
2
Year of publication
2001
Database
ISI
SICI code
0736-6205(200108)31:2<355:DOSSRM>2.0.ZU;2-E
Abstract
Simple sequence repeats (SSRs) were isolated from pearl millet bacterial ar tificial clones (BACs) without any subcloning steps. SSR sequences were tar geted using 3 ' end-anchored SSR primers. Flanking sequences were isolated by suppression PCR. In this pilot study 25 SSR markers have been developed from 40 BAC pools, comprising a total of 384 clones. This novel way to deve lop new markers has the added advantage that mapping the SSR markers will a nchor individual BACs to the genetic maps and, thus, facilitate the constru ction of BAC contigs.