H. Fukada et al., Development and validation of chemiluminescent immunoassay for vitellogenin in five salmonid species, COMP BIOC A, 130(1), 2001, pp. 163-170
Citations number
22
Categorie Soggetti
Animal Sciences",Physiology
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
A highly sensitive and specific chemiluminescent immunoassay (CLIA) was dev
eloped for quantification of vitellogenin (Vg) in five salmonids. The CLIA
for salmon Vg was performed using the two-site method, with anti-masu salmo
n beta ' -component as primary antibody and chemiluminescent acridinium-lab
eled anti-rainbow trout lipovitellin F(ab)' (2) as the second antibody. Usi
ng cutthroat trout Vg as the standard, the working range of the CLIA was fr
om 60 pg to 500 ng Vg/ml. Intra- and inter-assay coefficients of variation
ranged from 3.04 to 6.67% and 3.23 to 5.86%, respectively. For the various
salmonid species, serially diluted samples of serum from vitellogenic fish
ran parallel to their purified Vg standard curve in the CLIA. In male cutth
roat trout maturing during the 4 months before spawning, serum Vg levels ra
nged from 1.56 to 8000 ng/ml. High levels of Vg in some individuals may hav
e resulted from temporary elevation of estradiol-17 beta levels in the same
fish during December or January (1-2 months before spawning). This is the
first report on changes in serum Vg levels in maturing male trout using CLI
A, the most sensitive assay for Vg yet developed. (C) 2001 Elsevier Science
Inc. All rights reserved.