Engineered metalloproteins offer interesting systems for electrochemical st
udies of protein structure/function and their applications in nanobiotechno
logy. Scanning probe microscopy and cyclic voltammetry of engineered metall
oproteins and electrodes have proved to be a powerful combination of tools
contributing to the field of bioelectrochemistry. The ability to engineer t
ags, such as histidine tags and biotin-acceptor peptides, and to site-speci
fically introduce cysteine residues enabled the creation of ordered immobil
ised protein structures that can be characterised both electrochemically an
d topographically. Gene fusion and de novo combinatorial synthesis of metal
loproteins are emerging to provide structures with the desired electrochemi
cal properties.