Imprinted expression of Neuronatin from modified BAC transgenes reveals regulation by distinct and distant enhancers

Neuronatin (Nnat) is an imprinted gene that is expressed exclusively from t he paternal allele while the maternal allele is silent and methylated. The Nnat locus exhibits some unique features compared with other imprinted doma ins. Unlike the majority of imprinted genes, which are organised in cluster s and coordinately regulated, Nnat does not appear to be closely linked to other imprinted genes. Also unusually, Nnat is located within an 8-kb intro n of the Bc10 gene, which generates a biallelically expressed, antisense tr anscript. A similar organisation is conserved at the human NNAT locus on ch romosome 20. Nnat expression is first detected at E8.5 in rhombomeres 3 and 5, and subsequently, expression is widespread within postmitotic neuronal tissues. Using modified BAC transgenes, we show that imprinted expression o f Nnat at ectopic sites requires, at most, an 80-kb region around the gene. Furthermore, reporter transgenes reveal distinct and dispersed cis-regulat ory elements that direct tissue-specific expression and these are predomina ntly upstream of the region that confers allele-specific expression. (C) 20 01 Academic Press.