H. Terada et al., Ontogenesis of neurons immunoreactive for nitric oxide synthase in rat forebrain and midbrain, DEV BRAIN R, 128(2), 2001, pp. 121-137
We studied the immunohistochemical localization of neuronal nitric oxide sy
nthase (nNOS) in the developing rat brain on embryonic days 13 (E13), 15 (E
15), 17 (E17) and 19 (E19) and postnatal days 0 (P0), 7 (P7) and 14 (P14).
A few neurons positive for nNOS were first detected at E15 in the hypothala
mus and pons. At E17, many positive cells became detectable in the thalamus
. At E19, the positive cells in these three regions were rapidly increased
in number, and a few positive neurons were also observed in such regions as
the cerebral cortex and striatum. Positive cells in the hypothalamus tende
d to locate ventrolaterally, Positive neurons, stained very intensely as in
adult rats, were seen in the pedunculopontine tegmental nucleus, laterodor
sal tegmental nucleus and parafascicular nucleus. Two weeks after birth, po
sitive neurons of larger somata with many processes were distributed widely
in the cerebral cortex and hippocampus. The present study indicates that,
in the forebrain and midbrain, the distribution pattern of nNOS-containing
neurons is fundamentally completed by E19. (C) 2001 Elsevier Science BY All
rights reserved.