Binding interactions between barley thaumatin-like proteins and (1,3)-beta-D-glucans - Kinetics, specificity, structural analysis and biological implications

The specificity and kinetics of the interaction between the pathogenesis-re lated group of thaumatin-like proteins (PR5) in higher plants and (1,3)-bet a -D-glucans have been investigated. Two thaumatin-like proteins with 60% a minoacid sequence identity were purified from extracts of germinated barley grain, and were designated HvPR5b and HvPR5c. Purified HvPR5c interacted w ith insoluble (1,3)-beta -D-glucans, but not with cellulose, pustulan, xyla n, chitin or a yeast mannoprotein. Tight binding was observed with unbranch ed and unsubstituted (1,3)-beta -D-glucans, and weaker binding was seen if (1,6)-beta -linked branch points or beta -glucosyl substituents were presen t in the substrate. The HvPR5b protein interacted weakly with insoluble (1, 3)-beta -D-glucans and did not bind to any of the other polysaccharides tes ted. This indicated that only specific barley PR5 isoforms interact tightly with (1,3)-beta -D-glucans. The complete primary structures of HvPR5b and HvPR5c were determined and used to construct molecular models of HvPR5b and HvPR5c, based on known three-dimensional structures of related thaumatin-l ike proteins. The models were examined for features that may be associated with (1,3)-beta -D-glucan binding, and a potential (1,3)-beta -D-glucan-bin ding region was located on the surface of HvPR5c. No obvious structural fea tures that would prevent binding of (1,3)-beta -D-glucan to HvPR5b were ide ntified, but several of the amino acids in HvPR5c that are likely to intera ct with (1,3)-beta -D-glucans are not present in HvPR5b.