Increased levels of sigJ mRNA in late stationary phase cultures of Mycobacterium tuberculosis detected by DNA array hybridisation

In order to determine which genes are involved in maintaining viability of 100-day stationary-phase bacteria and persistent bacteria after antibiotic treatment, we used a mini-DNA array to examine the transcription of 82 gene s of M. tuberculosis in the 100-day stationary-phase cultures before and af ter rifampicin treatment. We found that the mRNA level of a sigma factor ge ne, sigJ, was strongly up-regulated in the late stationary-phase cultures. Other genes were also up-regulated, although to a lesser extent than sigJ. Surprisingly, after rifampicin treatment there was no significant change in sigJ expression, and most of the other 82 genes in the mini-DNA array also maintained expression, some at relatively high levels. These results sugge st that SigJ may control gene expression in the quiescent state and may be an important component in the mechanisms by which M. tuberculosis survives prolonged stationary phase even in the presence of sterilising antibiotics. (C) 2001 Federation of European Microbiological Societies. Published by El sevier Science B.V. All rights reserved.