Pregnancy and delivery after cryopreservation of zygotes produced by in-vitro matured oocytes retrieved from a woman with polycystic ovarian syndrome

This case report describes the birth of a healthy infant after cryopreserva tion of zygotes produced by in-vitro matured oocytes retrieved from an anov ulatory woman with polycystic ovarian syndrome (PCOS). To initiate the trea tment cycle, the patient received intravaginal progesterone at night for 10 days to induce a withdrawal bleed. Oocyte retrieval was performed on day 1 1 following a withdrawal bleed. The patient was administered 10 000 IU of H CG subcutaneously 36 h prior to oocyte collection. A total of 63 immature o ocytes were obtained; 10 were morphologically abnormal. Following incubatio n for 24-48 h in the maturation medium, TC-199 supplemented with 20% patien t's own serum, 75 mIU/ml FSH and LH, 77.4% (41/53) of the oocytes were at t he metaphase-II stage. Thirty-one (31/41, 75.6%) were fertilized using ICSI with her husband's spermatozoa, 15 fertilized oocytes were cultured for em bryo transfer and 16 were frozen at the pronuclear stage. Pregnancy ensued following fresh embryo transfer. Unfortunately, the pregnancy was miscarrie d eight weeks later. However, the second frozen-thawed embryo transfer atte mpt resulted in a full-term pregnancy with delivery of a healthy male infan t.