S. Erturk et al., Determination of vigabatrin in human plasma and urine by high-performance liquid chromatography with fluorescence detection, J CHROMAT B, 760(2), 2001, pp. 207-212
A sensitive and specific HPLC method has been developed for the assay of vi
gabatrin in human plasma and urine. The assay involves derivatization with
4-chloro-7-nitrobenzofurazan, solid-phase extraction on a silica column and
isocratic reversed-phase chromatography with fluorescence detection. Aspar
tam. was used as an internal standard. The assay was linear over the concen
tration range of 0.2-20.0 mug/ml for plasma and 1.0-15.0 mug/ml for urine w
ith a lower limit of detection of 0.1 mug/ml using 0.1 ml of starting volum
e of the sample. Both the within-day and day-to-day reproducibilities and a
ccuracies were less than 5.46% and 1.6%, respectively. After a single oral
dose of 500 mg of vigabatrin, the plasma concentration and the cumulative u
rinary excretion of the drug were determined. (C) 2001 Elsevier Science B.V
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