Preparative isolation and purification of phenylethanoid glycosides from the extract of faeces of beagle dogs by high-speed countercurrent chromatography

Authors
Lei, L Yang, FQ Zhang, TY Tu, PF Wu, LJ Chen, FK Ito, Y
Citation
L. Lei et al., Preparative isolation and purification of phenylethanoid glycosides from the extract of faeces of beagle dogs by high-speed countercurrent chromatography, J LIQ CHR R, 24(14), 2001, pp. 2187-2195
Citations number
4
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
10826076 → ACNP
Volume
24
Issue
14
Year of publication
2001
Pages
2187 - 2195
Database
ISI
SICI code
1082-6076(2001)24:14<2187:PIAPOP>2.0.ZU;2-7
Abstract
High-speed countercurrent chromatography (HSCCC) was applied to the separat ion of acteoside and isoacteoside from a crude extract from canine faeces a fter the dogs were orally administered phenylethanoid glycosides (PhGs). Tw o-step separation with a quaternary two-phase solvent system composed of et hyl acetate-n-butanol-ethanol-water (system 1, 35:6:6:50, v/v; system 2, 30 :10:6:50, v/v) was used. HPLC analysis of final CCC fractions revealed that the two main PhGs showed purity of over 95%. Their chemical structures wer e identified by (HNMR)-H-1, (CNMR)-C-13, FAB-MS.