Evidence of an inhibitory effect of diet and exercise on prostate cancer cell growth

Purpose: A high fat diet and sedentary lifestyle may predispose men to pros tate cancer through effects on serum factors such as hormones. We evaluated the effects of a low fat, high fiber diet and exercise intervention on ser um stimulated growth of established prostate cancer cell lines. Materials and Methods: Fasting serum was obtained from 13 overweight men be fore and after undergoing an 11-day low fat, high fiber diet and exercise i ntervention. Serum was also obtained from 8 men who had complied with the r egimen for a mean of 14.2 years. Hormone dependent LNCaP and independent PC -3 prostate cancer cell lines were grown in culture medium containing 10% o f subject pre-intervention or post-intervention serum and viable cells were counted after 48 hours. Anthropometry, serum free testosterone, lipids and glucose were measured in all subjects. Results: Post-intervention serum from each of the 11-day intervention subje cts reduced LNCaP cell growth by a mean of 30% compared with pre-interventi on serum from each (p < 0.01). LNCaP cell growth in serum from long-term su bjects was 15% below that of post-intervention serum (p < 0.01). There was no difference in the growth of PC-3 cells when cultured with serum from eit her intervention group. Serum free testosterone, body weight, glucose and l ipids were significantly reduced in 11-day subjects. Conclusions: A low fat, high fiber diet and exercise intervention resulted in serum changes that significantly reduced the growth of androgen responsi ve LNCaP prostate cancer cells in vitro.