A. Kotsakis et al., Microtubule reorganization during herpes simplex virus type 1 infection facilitates the nuclear localization of VP22, a major virion tegument protein, J VIROLOGY, 75(18), 2001, pp. 8697-8711
Full-length VP22 is necessary for efficient spread of herpes simplex virus
type 1 (HSV-1) from cell to cell during the course of productive infection.
VP22 is a virion phosphoprotein, and its nuclear localization initiates be
tween 5 and 7 h postinfection (hpi) during the course of synchronized infec
tion. The goal of this study was to determine which features of HSV-1 infec
tion function to regulate the translocation of VP22 into the nucleus. We re
port the following. (i) HSV-1 (F)-induced microtubule rearrangement occurre
d in infected Vero cells by 13 hpi and was characterized by the loss of obv
ious microtubule organizing centers (MtOCs). Reformed MtOCs were detected a
t 25 hpi. (ii) VP22 was observed in the cytoplasm of cells prior to microtu
bule rearrangement and localized in the nucleus following the process. (iii
) Stabilization of microtubules by the addition of taxol increased the accu
mulation of VP22 in the cytoplasm either during infection or in cells expre
ssing VP22 in the absence of other viral proteins. (iv) While VP22 localize
d to the nuclei of cells treated with the microtubule depolymerizing agent
nocodazole, either taxol or nocodazole treatment prevented optimal HSV-I(F)
replication in Vero cells. (v) VP22 migration to the nucleus occurred in t
he presence of phosphonoacetic acid, indicating that viral DNA and true lat
e protein synthesis were not required for its translocation. Based on these
results, we conclude that (iv) microtubule reorganization during HSV-1 inf
ection facilitates the nuclear localization of VP22.