We studied deletion and monosomy of chromosome 7 in 150 patients with myelo
proliferative diseases. We found 8/150 patients with monosomy 7 by cytogene
tics and 4/150 with deletions of the long arm of chromosome 7 by restrictio
n fragment length polymorphism (RFLP) analysis performed with Southern and
polymerase chain reaction. To overcome limitation of RFLP analysis, we rest
ricted loss of heterozygosity study with microsatellites to 45 patients, ob
serving deletion 7q31.1 in 7/45 patients. In all patients with molecular al
terations the deletion was observed only in myeloid cells, while the monoso
my was detected in both myeloid precursor and lymphocytes. This finding sug
gests a CD34-totipotent stem cell origin for the monosomy and a colony form
ing unit - granulocyte, erytrocyte, monocyte, megakaryocytes (CFU-GEMM) ste
m cell origin for the deletions. (C) 2001 Elsevier Science Ltd. All rights
reserved.