I. Vorobtsova et al., An investigation of the age-dependency of chromosome abnormalities in human populations exposed to low-dose ionising radiation, MECH AGE D, 122(13), 2001, pp. 1373-1382
Among various cytogenetic changes stable chromosome aberrations (SCHA) seem
to be the most significant for ageing and carcinogenesis. Being nonlethal
they can persist through cell divisions and accumulate in time. We studied
the age response of SCHA (translocations and insertions) in normal and radi
ation exposed human populations. Two cohorts of people at the age range of
3-72 years were studied: control (43 persons) and exposed to low doses of a
ccidental irradiation due to Chernobyl accident and atomic bomb testing in
Semipalatinsk (67 persons). FISH method was used for visualisation of chrom
osome aberrations. Metaphases from cultured lymphocytes were hybridised wit
h biotinilated whole chromosome specific DNA probes for 1, 4 and 12 chromos
omes, and with pancentromeric probe labelled with digoxigenin. The frequenc
y of SCHA in lymphocytes increased as a quadratic function of donor age in
both populations studied, being higher in exposed cohort as compared with c
ontrol one. No age dependence for dicentrics was observed. The frequency of
SCHA is a reliable biomarker of ageing in humans. Quadratic model of their
age-response gives reasons to suggest that their increase is due to lower
level of DNA repair or/and the genomic instability in older people. The exp
osure of people to low doses of ionising radiation accelerates the age-rela
ted increase of SCHA frequency. (C) 2001 Elsevier Science Ireland Ltd. All
rights reserved.