Pharmacokinetics and tissue distribution of piperine in animals after iv. bolus administration

A reverse phase HPLC method to determine piperine, a pungent constituent of black pepper, in rabbit serum and various tissues of the rat was developed . A pharmacokinetic study was performed in rabbits and tissue distribution studies were carried out in rats. High reproducibility was achieved in quan titative analysis over the concentration range of 0.2-20 mug/ml serum. Afte r bolus intravenous administration of piperine at a dose of 10 mg/kg, the s erum concentration - time curve fitted the two-compartment open model. The tissue distribution pattern of pipeline in rats also supports the two-compa rtment open model.