HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROSPRAY-IONIZATION TANDEMMASS-SPECTROMETRY (HPLC-ESI-MS-MS) FOR THE QUANTIFICATION OF L-KYNURENINE AND INDOLE-3-ACETIC-ACID IN GRAPE MUST BY ISOTOPE-DILUTION ASSAY

Authors
DOLLMANN B RICHLING E HERDERICH M KOHLER H SCHWAB A SCHMITT A SCHREIER P
Citation
B. Dollmann et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROSPRAY-IONIZATION TANDEMMASS-SPECTROMETRY (HPLC-ESI-MS-MS) FOR THE QUANTIFICATION OF L-KYNURENINE AND INDOLE-3-ACETIC-ACID IN GRAPE MUST BY ISOTOPE-DILUTION ASSAY, Vitis, 36(2), 1997, pp. 97-101
Citations number
12
Categorie Soggetti
Agriculture
Journal title
Vitis → ACNP
ISSN journal
00427500
Volume
36
Issue
2
Year of publication
1997
Pages
97 - 101
Database
ISI
SICI code
0042-7500(1997)36:2<97:HLET>2.0.ZU;2-Z
Abstract
Isotope dilution assay was used for the qualitative and quantitative a nalysis of L-kynurenine and indole-3-acetic acid in grape must. After solid phase extraction, highest selectivity and sensitivity was achiev ed by high performance liquid chromatography-electrospray ionization t andem mass spectrometry (HPLC-ESI-MS-MS) using selected reaction monit oring (SRM). In the 24 samples under study, the amounts of L-kynurenin e and indole-3-acetic acid ranged from 0 to 94 mu g.l(-1) and from 20 to 380 mu g.l(-1), respectively. These compounds are considered as pot ential precursors of 2-aminoacetophenone, causing the ''untypical agin g off-flavour'' in Vitis vinifera white wines.