A rapid and quantitative mass spectrometry method for determining the concentration of acylcarnitines and aminoacids in amniotic fluid

Authors
Braida, L Crovella, S Boniotto, M Luchesi, A de Vonderweid, U Casetta, B Amoroso, A
Citation
L. Braida et al., A rapid and quantitative mass spectrometry method for determining the concentration of acylcarnitines and aminoacids in amniotic fluid, PRENAT DIAG, 21(7), 2001, pp. 543-546
Citations number
14
Categorie Soggetti
Reproductive Medicine","Medical Research Diagnosis & Treatment
Journal title
PRENATAL DIAGNOSIS
ISSN journal
01973851 → ACNP
Volume
21
Issue
7
Year of publication
2001
Pages
543 - 546
Database
ISI
SICI code
0197-3851(200107)21:7<543:ARAQMS>2.0.ZU;2-O
Abstract
We describe a quantitative. rapid, sensitive and reproducible tandem mass s pectrometry (MSMS) method for the one-step detection of aminoacid (AAs) and acylcarnitine (ACs) concentrations in amniotic fluid. This technology is q uicker and more sensitive than other methods used to date since it is possi ble to determine very low AA and AC concentrations in samples simultaneousl y in a single run. The high degree of automation allows a large number of p regnancies to be screened for metabolic defects in a very short time. Copyr ight (C) 2001 John Wiley & Sons, Ltd.