A highly specific one-step PCR-assay for the rapid discrimination of enteropathogenic Yersinia enterocolitica from pathogenic Yersinia pseudotuberculosis and Yersinia pestis

Authors
Arnold, T Hensel, A Hagen, R Aleksic, S Neubauer, H Scholz, HC
Citation
T. Arnold et al., A highly specific one-step PCR-assay for the rapid discrimination of enteropathogenic Yersinia enterocolitica from pathogenic Yersinia pseudotuberculosis and Yersinia pestis, SYST APPL M, 24(2), 2001, pp. 285-289
Citations number
29
Categorie Soggetti
Microbiology
Journal title
SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN journal
07232020 → ACNP
Volume
24
Issue
2
Year of publication
2001
Pages
285 - 289
Database
ISI
SICI code
0723-2020(200107)24:2<285:AHSOPF>2.0.ZU;2-H
Abstract
Based on differences within the yopT-coding region of Yersinia. enterocolit ica, Y. pseudotuberculosis and Y pestis, a rapid and sensitive one-step pol ymerase chain reaction assay with high specificity for pathogenic Y enteroc olitica was developed. By this method pathogenic isolates of Y. enterocolit ica can be easily identified and discriminated from other members of this g enus. The entire coding sequence of the yopT effector gene of Y. pseudotube rculosis Y36 was determined.