MAINTENANCE OF STEROID 11-HYDROXYLATION ACTIVITY IN IMMOBILIZED CUNNINGHAMELLA-ELEGANS PROTOPLASTS

Authors
DLUGONSKI J PARASZKIEWICZ K SEDLACZEK L
Citation
J. Dlugonski et al., MAINTENANCE OF STEROID 11-HYDROXYLATION ACTIVITY IN IMMOBILIZED CUNNINGHAMELLA-ELEGANS PROTOPLASTS, World journal of microbiology & biotechnology, 13(4), 1997, pp. 469-473
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
World journal of microbiology & biotechnology → ACNP
ISSN journal
09593993
Volume
13
Issue
4
Year of publication
1997
Pages
469 - 473
Database
ISI
SICI code
0959-3993(1997)13:4<469:MOS1AI>2.0.ZU;2-3
Abstract
Cunninghamella elegans protoplasts entrapped in calcium alginate at ge l concentration of 6% w/v maintained cortexolone 11 alpha- and 11 beta -hydroxylation activity for 13 cycles, and a total time of 247 h, in t he presence of organic osmotic stabilizers and cell wall regeneration inhibitors. Changes in stabilization medium composition showed that it is possible to stimulate separately epihydrocortisone and hydrocortis one production in immobilized C. elegans protoplasts.