THE EFFECT OF FREEZE-THAWING ON THE RELEASE OF INTRACELLULAR PROTEINSFROM ESCHERICHIA-COLI BY MEANS OF A BEAD MILL

Authors
BAKIR U HAMAMCI H
Citation
U. Bakir et H. Hamamci, THE EFFECT OF FREEZE-THAWING ON THE RELEASE OF INTRACELLULAR PROTEINSFROM ESCHERICHIA-COLI BY MEANS OF A BEAD MILL, World journal of microbiology & biotechnology, 13(4), 1997, pp. 475-477
Citations number
7
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
World journal of microbiology & biotechnology → ACNP
ISSN journal
09593993
Volume
13
Issue
4
Year of publication
1997
Pages
475 - 477
Database
ISI
SICI code
0959-3993(1997)13:4<475:TEOFOT>2.0.ZU;2-G
Abstract
Escherichia coli cell pastes were freeze-thawed prior to milling, to d etermine its effect on the efficiency of the bead mill. The quantity o f the released proteins and the extracted activity of a periplasmic en zyme, penicillin acylase (EC 3.5.1.11), increased with the application of freeze-thawing. Freezing, but not the frozen storage that follows was the main reason for increased bead mill efficiency.