Preparation of (TcN)-Tc-99m(CBDTC)(2) and its biodistribution in mice

In order to seek a new class of brain perfusion imaging agent containing [9 9(m)TcN](2+) core, bis(N-cyclobutyl dithiocarbamato) nitrido technetium-99m complex [(99m)-TcN(CBDTC)(2)] (CBDTC: N-cyclobutyl dithiocarbamato) was sy nthesized through a simple and efficient method, which can be utilized for the preparation of a radiopharmaceutical through a lyophilized kit formulat ion. The radiochemical purity of the complex was over 90%, as measured by t hin layer chromatography. No decomposition of the complex at room temperatu re was observed over a period of 6 h. It's partition coefficient indicated that it is a good lipophilic complex. Biodistribution in mice showed that t he complex was significantly retained in the brain. The brain uptake (ID %/ g) was 3.61, 3.15 and 2.62 and the brain/blood ratio was 1.00, 1.44 and 1.3 0 at 5, 30 and 60 min post-injection, respectively. These results suggest t hat this compound could be a potential brain perfusion imaging agent. (C) 2 001 Elsevier Science Ltd. All rights reserved.