Colon cancer cell adhesion to endothelial E-selectin inhibits detachment of endothelial cells through activation of beta(1)-integrin

Previous studies have implicated a role for E-selectin in carcinoma cell ad hesion to vascular endothelium. We examined the role of colon cancer cell a dhesion to vascular endothelium via E-selectin using adenoviral vector-medi ated transfection in human umbilical vein endothelial cells (HUVECs). We fo und that the amount of HUVEC detachment from the gelatin matrix 24 h after LS-180 cell adhesion was inhibited only when the HUVECs were transduced wit h wild-type E-selectin, but not with a cytoplasmic domain truncated mutant E-selectin or the control Lac-Z vector. We also found that the adhesion of LS-180 cells to wild-type E-selectin transduced HUVEC-induced activation of beta (1)-integrin receptors without affecting MMP activity. These results indicate that colon cancer cell adhesion via E-selectin inhibits HUVEC deta chment from the monolayer, at least in part by modulating beta (1)-integrin activity in HUVECs. In addition, they indicate the importance of the cytop lasmic domain of E-selectin with this phenomenon. (C) 2001 Academic Press.