Kinetics study of a selenium-containing ScFv catalytic antibody that mimics glutathione peroxidase

Authors
Su, D You, DL Ren, XJ Luo, GM Mu, Y Yan, GL Xue, Y Shen, JC
Citation
D. Su et al., Kinetics study of a selenium-containing ScFv catalytic antibody that mimics glutathione peroxidase, BIOC BIOP R, 286(1), 2001, pp. 189-194
Citations number
35
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
286
Issue
1
Year of publication
2001
Pages
189 - 194
Database
ISI
SICI code
0006-291X(20010810)286:1<189:KSOASS>2.0.ZU;2-L
Abstract
The steady-state kinetics study and some enzymatic characterization of a se lenium-containing scFv catalytic antibody (Se-scFv2F3) were carried out. A novel reaction formula of this abzyme-catalyzed reaction was proposed and a rate equation was obtained according to the formula. The constants in the equation were compared with Dalziel's parameters and the exact meanings of these constants were analyzed. The obtained kinetics parameters from the ki netics study of Se-scFv2F3 were analyzed and compared with those of native glutathione peroxidase. (C) 2001 Academic Press.