Novel amidohydrolytic reactions in oxidative pyrimidine metabolism: Analysis of the barbiturase reaction and discovery of a novel enzyme, ureidomalonase

Authors
Soong, CL Ogawa, J Shimizu, S
Citation
Cl. Soong et al., Novel amidohydrolytic reactions in oxidative pyrimidine metabolism: Analysis of the barbiturase reaction and discovery of a novel enzyme, ureidomalonase, BIOC BIOP R, 286(1), 2001, pp. 222-226
Citations number
9
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
286
Issue
1
Year of publication
2001
Pages
222 - 226
Database
ISI
SICI code
0006-291X(20010810)286:1<222:NARIOP>2.0.ZU;2-8
Abstract
Amidohydrolytic reactions in oxidative pyrimidine metabolism were investiga ted in detail. Barbiturase has been reported to catalyze the amidohydrolysi s of barbituric acid to urea and malonic acid. However, purification of the enzyme revealed that it catalyzes the ring-opening of barbituric acid to u reidomalonic acid. The existence of a consecutive enzyme named ureidomalona se, which hydrolyzes ureidomalonic acid to urea and malonic acid, was also discovered during the purification of barbiturase. (C) 2001 Academic Press.