Rainbow trout (Onchorhynchus mykiss) hepatic glucose transporter

We report identification of a rainbow trout hepatic glucose transporter sha ring 58% and 52% amino acid identity with avian and mammalian GLUT2 sequenc es, respectively. The functionality of OnmyGLUT2 was assessed by expression in rainbow trout embryos. We also measured the transport of hexose in isol ated rainbow trout hepatocytes. Inhibition of 3-O-methylglucose uptake by c ytochalasin B, phloretin and 2-deoxy-D-glucose suggested the existence of a functional facilitative transporter in these cells. Expression of OnmyGLUT 2 was found in the liver, kidney and intestine. (C) 2001 Elsevier Science B Y. All rights reserved.