Study on gelatin-containing artificial skin IV: a comparative study on theeffect of antibiotic and EGF on cell proliferation during epidermal healing

Gelatin-hyaluronate sponge with and without antibiotic and epidermal growth factor (EGF) were prepared and compared. Four types of sponges were applie d on the full-thickness dorsal skin defect of Wistar rat. The effects of an tibiotic and EGF in gelatin-hyaluronate sponge on wound healing were invest igated by light microscopy and image analyzer at postoperative days of 5, 1 2 and 21. An immunohistochemical technique, employing PC10, a monoclonal an tibody against proliferating cell nuclear antigen (PCNA) was applied to wou nded tissue sections. The number of PC10-positive cells was very high for t he sponge with EGF at postoperative day 5, then gradually decreased with ti me. Also we found that antibiotics restrained the cell proliferation during the migratory phase. The sponge with both antibiotic and EGF showed good w ound healing performances on the whole for a healing period. The epithelium was regenerated fast with EGF-impregnated sponges at day 5, but each sampl e had nearly the same length of regenerated epithelium at day 12. (C) 2001 Elsevier Science Ltd. All rights reserved.