Immunohistochemically detected micrometastases of the lymph nodes in patients with gastric carcinoma

BACKGROUND. Lymph node status is a major determinant of disease recurrence after patients undergo curative resection for gastric carcinoma. A proporti on of patients without lymph node metastasis develop systemic recurrences. Recent studies in a range of solid tumors have found a high incidence of mi crometastases in the regional lymph nodes of patients with apparently negat ive lymph nodes. In patients with breast and colorectal carcinoma, the pres ence of micrometastases has been associated with a poorer prognosis. In pat ients with gastric carcinoma, the significance of micrometastases in lymph nodes remains controversial. Most published reports on this subject suffer from the problems of small sample size and selection bias. METHODS. One hundred seven patients with pathologic T2N0M0 (tumor invades m uscularis propria or subserosa [T2], no regional lymph node metastasis [NO] , and no distant metastasis [MO]; pT2N0M0) gastric carcinoma who underwent gastric resection between 1984 and 1990 at the National Cancer Center Hospi tal were studied. Two consecutive sections were newly prepared from each ly mph node for hematoxylin and eosin staining and immunohistochemical stainin g (IHC) with antibody against cytokeratin. Associations between clinicopath ologic factors and the presence of micrometastases as well as micrometastas es and survival were sought. RESULTS. Micrometastases were identified in 38 of 107 patients (35.5%) and in 87 of 4484 lymph nodes (1.94%) by IHC. The incidence of micrometastases was significantly higher in patients with infiltrative tumors than in patie nts with expansive, growing tumors (P = 0.02). Other clinicopathologic find ings had no statistically significant correlation with the incidence of mic rometastases. The 5-year survival rates of patients with and without microm etastases were 94% and 89%, respectively. Similarly, the 10-year survival r ates were 79% and 74%, respectively. The survival curves of patients with o r without micrometastasis were nearly superimposed (P = 0.86). CONCLUSIONS. The presence of immunohistochemically detected micrometastases in the regional lymph nodes did not affect the survival of Japanese patien ts with pT2N0M0 gastric carcinoma who had undergone gastrectomy with D2 lym ph node dissection. (C) 2001 American Cancer Society.