BACKGROUND. Neuroblastomas (NBs) almost ubiquitously express the gangliosid
e GD2. GD2 synthesis is dependent on the key enzyme GD2 synthase. Thus, GD2
synthase transcript may prove to be a potential molecular marker of NB.
METHODS. Seventy-seven NB tumor tissues of all stages, 5 NB cell tines, and
26 normal bone marrows (BMs) and peripheral blood (PBL) samples, as well a
s 26 non-NB remission-Bids were analyzed for the expression of GD2 synthase
by a highly sensitive reverse transcriptase-polymerise chain reaction (RT-
PCR) and chemiluminescence detection. One hundred fifty-two NB BMs were tes
ted and comparisons were made among three independent detection techniques,
namely GD2 synthase RT-PCR, immunofluorescence (IF), and histology (HIST).
RESULTS. GD2 synthase transcript was present in 5 of 5 cell lines and in 77
of 77 tumors tested. Among 116 marrows that were positive by at least 1 of
the 3 methods, 78% were detectable by GD2 synthase, 68% by IF, and 46% by
HIST. Seventy-six percent of positive BMs that were obtained during treatme
nt and follow-up had GD2 synthase expression, whereas only 29% were HIST po
sitive. Correlation between RT-PCR and IF was high (P = 0.001), and positiv
ity by 3 out of 3 methods was strongly correlated with poor survival (P < 0
.01). Of note, marrows tested at the time of chemotherapy were positive by
at least 2 out of 3 methods and were associated with adverse outcome (P = 0
.01). Serial samples (n = 28) in 5 patients demonstrated close agreement be
tween RT-PCR and patient disease status.
CONCLUSIONS. The current study found that molecular detection of GD2 syntha
se transcript in NB BMs may have potential value in detecting rare tumor ce
lls. (C) 2001 American Cancer Society.