K. Sato et al., Mass spectrometric high-throughput analysis of serum-free conditioned medium from cancer cell lines, CANCER LETT, 170(2), 2001, pp. 153-159
Proteins secreted by cancer cells may be useful as tumor markers. We analyz
ed constituents produced by cells grown in serum-free conditioned medium to
avoid confounding factors present in serum. Mass spectrometric techniques
were used to obtain expression patterns of constituents between 2000 and 15
000 Da from as little as I [il of crude sample. This protocol can produce
distinctive mass profiles from 16 cell lines within 1 h. Thus, differential
display by mass spectrometry will expedite the discovery of peptides speci
fically secreted by particular cells. These data illustrate the advantage o
f this strategy over the conventional approach of electrophoretic separatio
n of serum samples. (C) 2001 Elsevier Science Ireland Ltd. All rights reser
ved.