Loss of Annexin II heavy and light chains in prostate cancer and its precursors

Annexin II mRNA coding for a calcium binding protein was found to be absent in prostate cancer by subtractive hybridization and Northern analysis. In contrast to high expression in normal and benign hyperplastic glandular and basal epithelium, Annexin II heavy (p36) and light (p11) chains in 31/31 p rostate cancer specimens were lost immunohistochemically. In glands involve d by prostate intraepithelial neoplasia, 65% lost both chains in glandular epithelial cells, whereas basal cells were all positively stained. Southern analysis of cancer DNA showed no noticeable deletion in p36 gene. LNCaP ce lls treated with 5-azacytidine re-expressed p36, suggesting methylation cou ld be responsible for the silencing.