Studies on the cleavage of bovine serum albumin by Ser-His

Authors
Chen, J Wan, R Liu, H Jiang, YY Zhao, YF
Citation
J. Chen et al., Studies on the cleavage of bovine serum albumin by Ser-His, CHEM J CH U, 22(8), 2001, pp. 1349-1351
Citations number
12
Categorie Soggetti
Chemistry
Journal title
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN journal
02510790 → ACNP
Volume
22
Issue
8
Year of publication
2001
Pages
1349 - 1351
Database
ISI
SICI code
0251-0790(200108)22:8<1349:SOTCOB>2.0.ZU;2-F
Abstract
It has been found that Ser-His could cleave bovine serum albumin (BSA), wit h the most suitable pH around 6. 0. The kinds of buffers have different inf luences on the cleavage activity. The phosphate buffer and BR buffer are th e most effective. The hydroxyl group of the serine residue is the essential functional group for the cleavage, while the amide bond, imidazole, and ca rboxyl group of Ser-His increase the cleavage activity.