Studies on the kinetic properties of cells of aquatic animals during deathwith P-31 NMR

Cell-death manner is related to the contents of intra-cell energy substance s. The rate constants and the half life-time of ATP, phosphocreatine (PCr) and inorganic phosphorus (P-i) in different cells of eel and crucian during their death have been determined with P-31 NMR. It has been found that the re are differences in rate constants and change rule of these energy substa nces for different animals and different cells in the same animal from this study. The decreasing order of the half life-time is: muscle of eel > brai n of eel > muscle of crucian for ATP; brain of eel approximate to muscle of eel > muscle of crucian for PCr. These results are very vital to understan ding the concerned change pattern of energy substances and the speed of cel l-death.