Studies on high-resolution hadamard transform microscopic image detection system using one-dimensional mechanical mask

A novel Hadamard transform(HT) microscopic image detection system which can generate 511 x 512 pixel format images of fluorescence or absorptive light for small samples has been developed, when spatial multiplexing is perform ed with a 511-slit one-dimensional Hadamard mask and a highly sensitive lin ear CCD. The weak native fluorescence imaging for Zephyranthes candida(Lind l.) Herb. Pollen cells can be realized within 1 min. The spatial resolution of 0.6 mum per pixel has been achieved with a 40X objective. The imaging s peed, sensitivity and spatial resolution are satisfactory, hence the system has a good application prospect in cell biology and medicine. Some factors that influence imaging speed and quality have been discussed preliminarily .