A CD and FT-Raman study on the secondary structure of saporin-S6

Authors
Wang, YT Huang, PW Li, ZQ Xu, F Meng, QF Yang, Y Shi, XH Li, QS
Citation
Yt. Wang et al., A CD and FT-Raman study on the secondary structure of saporin-S6, CHEM J CH U, 22(7), 2001, pp. 1117-1119
Citations number
15
Categorie Soggetti
Chemistry
Journal title
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN journal
02510790 → ACNP
Volume
22
Issue
7
Year of publication
2001
Pages
1117 - 1119
Database
ISI
SICI code
0251-0790(200107)22:7<1117:ACAFSO>2.0.ZU;2-G
Abstract
Saporin-S6 is purified from the seeds of Saponaria officinalis and its CD a nd FT-Raman spectra are determined. From the CD spectra of fresh protein aq ueous and the same aqueous that was laid aside for 90 days, it is proved th at saporin-S6 has unusual stability. Throughout the FT-Raman spectra of sap orin-S6, we work out that the alpha -helix of saporin-S6 is 26%, while beta -fold is 37%, and random coil is 37%.