Studies on tetra-substituted amino aluminum phthalocyanine as a new red-region substrate for mimetic peroxidase

Tetra-substituted amino aluminum phthalocyanine (TAAlPc) has been synthesiz ed and used for the first time as a new red region fluorescent substrate fo r the determination of hydrogen peroxide catalyzed by peroxidase or mimetic peroxidase. Under optimum conditions, the calibration graph has a linear r ange of 0.0-3.0 x 10(-7) mol/L H2O2 with a detection limit of 3.7 x 10(-9) mol/L. The feasibility of TAAlPc as a new promising red-region substrate in practical application has been proven in the determination of H2O2 in rain water. The proposed method can largely minimize the interference that resul ts from background fluorescence or scattering light and has a high analytic al sensitivity.