BIOTECHNOLOGICAL POTENTIAL OF P450 MONOOXYGENASES - HIGH-LEVEL PRODUCTION OF BOVINE CYTOCHROME P450C17 MONOOXYGENASE DURING MEDIUM CELL-DENSITY CULTURE OF A RECOMBINANT YEAST, SACCHAROMYCES-CEREVISIAE GRF-18 (YEP-TOKU1)

Authors
NISHIHARA H OKAMURA T SCHMID RD HAUCK A REUSS M
Citation
H. Nishihara et al., BIOTECHNOLOGICAL POTENTIAL OF P450 MONOOXYGENASES - HIGH-LEVEL PRODUCTION OF BOVINE CYTOCHROME P450C17 MONOOXYGENASE DURING MEDIUM CELL-DENSITY CULTURE OF A RECOMBINANT YEAST, SACCHAROMYCES-CEREVISIAE GRF-18 (YEP-TOKU1), Journal of biotechnology, 56(1), 1997, pp. 57-61
Citations number
20
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Journal of biotechnology → ACNP
ISSN journal
01681656
Volume
56
Issue
1
Year of publication
1997
Pages
57 - 61
Database
ISI
SICI code
0168-1656(1997)56:1<57:BPOPM->2.0.ZU;2-T
Abstract
Bovine cytochrome P450c17 monooxygenase was produced in a 30-L ferment er by Saccharomyces cerevisiae GRF18 (YEp-Toku1), harboring the GAL10 promoter, and using conditions of medium cell density culture. Upon ad dition of D-galactose as an inducer and FeCl3 as a cofactor, cells beg an to produce the P450 hemoprotein. The yield of this enzyme reached a maximum after 31 h but its formation continued for more than 60 h aft er induction. The amount of P450c17 produced was 4.7-fold as compared to shake flask experiments. (C) 1997 Elsevier Science B.V.