Lv. Stamm et al., IDENTIFICATION AND CHARACTERIZATION OF THE GYRB GENE FROM TREPONEMA-PALLIDUM SUBSP PALLIDUM, FEMS microbiology letters, 153(1), 1997, pp. 129-134
The nucleotide sequence of a DNA gyrase B subunit gene (gyrB) from Tre
ponema pallidum has been determined. Southern blot analysis of T. pall
idum chromosomal DNA indicated that this gene is present as a single c
opy. The organization of genes flanking the gyr B gene is unique in co
mparison to that of other bacteria. The gyrB gene encodes a 637 amino
acid protein whose deduced sequence has a high degree of homology with
type-II topoisomerase ATPase subunits (GyrB and ParE). Five type-II t
opoisomerase motifs, an ATP binding site (Walker A), and amino acid re
sidues that putatively interact with ATP, are highly conserved in the
T. pallidum GyrB protein.