NEOMYCIN-RESISTANCE AND SPECTINOMYCIN-RESISTANCE REPLACEMENT VECTORS FOR BACILLUS-SUBTILIS

Authors
CHARY VK AMAYA EI PIGGOT PJ
Citation
Vk. Chary et al., NEOMYCIN-RESISTANCE AND SPECTINOMYCIN-RESISTANCE REPLACEMENT VECTORS FOR BACILLUS-SUBTILIS, FEMS microbiology letters, 153(1), 1997, pp. 135-139
Citations number
14
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
153
Issue
1
Year of publication
1997
Pages
135 - 139
Database
ISI
SICI code
0378-1097(1997)153:1<135:NASRVF>2.0.ZU;2-I
Abstract
A plasmid is described for Bacillus subtilis that facilitates replacem ent of the widely used neomycin resistance gene (neo) with a spectinom ycin resistance (spc(E)) gene. A second plasmid is described that faci litates replacement of spc(S), associated with mini-Tn10 mutagenesis i n B, subtilis, with neo. These plasmids can also function as integrati ve vectors for B. subtilis. They expand the scope of strain constructi on and gene analysis in B. subtilis.