Pml. Dutra et al., Platelet-activating factor modulates a secreted phosphatase activity of the trypanosomatid parasite Herpetomonas muscarum muscarum, CURR MICROB, 43(4), 2001, pp. 288-292
In the present work we characterized the secreted phosphatase activity of t
he trypanosomatid parasite Herpetomonas muscarum muscarum. This housefly pa
rasite hydrolyzed p-nitrophenylphosphate at a rate of 10.26 nmol Pi/mg prot
ein/min. Classical inhibitors of acid phosphatases, such as sodium orthovan
adate (NaVO3), sodium fluoride (NaF), and ammonium molybdate promoted a dec
rease in this phosphatase activity. When the parasites were assayed in the
presence of sodium tartrate, an inhibitor of Leishmania spp-secreted acid p
hosphatases, this activity was drastically diminished. Cytochemical analysi
s showed the localization of this enzyme on the external surface and in the
flagellar pocket of these parasites. Sodium tartrate inhibited this reacti
on, confirming the biochemical data. Platelet-activating factor (PAF) inhib
ited the phosphatase activity determined in the supernatant of living H. m.
muscarum.