Structural studies of the Klentaq1 DNA polymerase

Authors
Citation
Y. Li et G. Waksman, Structural studies of the Klentaq1 DNA polymerase, CURR ORG CH, 5(8), 2001, pp. 871-883
Citations number
41
Categorie Soggetti
Organic Chemistry/Polymer Science
Journal title
CURRENT ORGANIC CHEMISTRY
ISSN journal
13852728 → ACNP
Volume
5
Issue
8
Year of publication
2001
Pages
871 - 883
Database
ISI
SICI code
1385-2728(200108)5:8<871:SSOTKD>2.0.ZU;2-D
Abstract
DNA polymerase I enzymes have served as model systems to study the mechanis m of template-directed DNA polymerization. This process requires that the e nzyme cycles through a series of conformational changes, each cycle leading to the incorporation of a nucleotide to the primer strand of the DNA. The kinetics of nucleotide incorporation has been extensively studied leading t o the definition of specific steps along the cycle. Efforts to visualize th ese steps using X-ray crystallography have recently come to fruition, notab ly for one particular DNA polymerase I system, that of Klentaql. This revie w focuses on the structural characterization of the various steps along the nucleotide incorporation pathway.