Isolation and sequence analysis of the arbuscular mycorrhizal fungus Glomus mosseae (Nicol & Gerd.) Gerdemann & Trappe 3-phosphoglycerate kinase (PGK) gene promoter region

The Glomus mosseae 3-phosphoglycerate kinase (GmPGK) gene promoter has been isolated from a phage genomic library and represents one of the few promot er elements to be isolated and analysed from these symbiotic fungi. The ana lysis revealed the presence of several motifs which are found in the promot er region of other fungal PGK genes. In particular, DNA sequences homologou s to segments of the S. cerevisiae and Rhizopus niveus upstream activating elements (UAS). The importance of these UAS sequences in regulating carbon source in PGK genes is known and the presence of two carbon source regulate d UAS sequences in the GmPGK gene promoter and its role in the biology of A M fungi is discussed briefly.