Mt. Matyska et al., Characterization and applications of etched chemically modified capillaries for open-tubular capillary electrochromatography, ELECTROPHOR, 22(12), 2001, pp. 2620-2628
In this article, the effects of the stationary phase, buffer pH, organic mo
difier type, organic modifier composition, applied voltage, and temperature
on the migration of several synthetic peptides in etched chemically modifi
ed open-tubular capillaries are discussed. With these solutes, migration is
due to two effects: electrophoretic mobility and solute/bonded phase inter
actions. In addition, relative migration rates are evaluated for the peptid
e samples as a function of these experimental variables in order to determi
ne which parameters might be useful for optimizing separations in open-tubu
lar capillary electrochromatography (OTCEC). Some examples of synthetic pep
tide separations are presented where the sample contains a major component
and several minor species, demonstrating how the resolution of these mixtur
es can be affected by the appropriate choice of experimental variables.